IL-1 Beta Rabbit Polyclonal Antibody

• Catalog Number: orb500658
• Category: Antibodies
• Description: IL-1 Beta Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Human, Insect, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-1 Beta (181-269/269aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17/32 kDa
• Target: IL1B
• UniProt ID: P01584
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Xuling et al. Effects of lysophosphatidic acid receptor 5 on NLRC4 inflammasome in brain tissues of transient cerebral ischemia/reperfusion rat Hum Exp Toxicol, 41, (2022)

PubMed: 35230166

Applications

WB

Reactivity

Rat

Sample: Hemocyte (Mouse) Lysate at 40 ug, Primary: Anti-IL-1 Beta (orb500658) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 30 kD.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-IL-1 Beta (orb500658) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17kD, Observed band size: 16kD.