IKK beta (phospho-Y199) antibody

• Catalog Number: orb214092
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IKBKB
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y199 of human IKK beta protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IKBKB
• Entrez: 3551, 16150, 84351
• UniProt ID: O88351, O14920, Q9QY78
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti IKKB antibody, anti Inhibitor of nuclear fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IKK beta (Phospho-Y199) expression in HeLa LPS-treated (A), Raw264.7 LPS-treated (B), PC12 LPS-treated (C) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 87 kD)

Immunohistochemical analysis of IKK beta (Phospho-Y199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.