IgM Heavy Chain Antibody [IM373]

Catalog Number: orb1252753

Catalog Number	orb1252753
Category	Antibodies
Description	IgM Heavy Chain Antibody [IM373]
Species/Host	Mouse
Clonality	Monoclonal
Clone Number	IM373
Tested applications	FC, IF, IHC, WB
Reactivity	Human
Isotype	IgG1, kappa
Immunogen	Recombinant heavy chain of human IgM was used as the immunogen for this antibody.
Concentration	0.2 mg/mL
Form/Appearance	Liquid
Conjugation	Unconjugated
Target	IGHM
UniProt ID	P01871
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Note	For research use only
Application notes	Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (Frozen): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date	12 months from date of receipt.

IHC testing of human tonsil stained with IgM heavy chain antibody.

Western blot testing of Raji cell lysate with IgM heavy chain antibody.

SDS-PAGE Analysis of Purified, BSA-Free IgM Heavy Chain Antibody (clone IM373). Confirmation of Integrity and Purity of the Antibody.