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IgG2b Isotype control (Biotin)
Catalog Number: orb154468
| Catalog Number | orb154468 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody conjugated to Biotin which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection. |
| Clonality | Monoclonal |
| Clone Number | MPC-11 |
| Tested applications | ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB |
| Isotype | Mouse IgG2b kappa |
| Immunogen | KLH-coupled trinitrophenol |
| Concentration | 1 mg/ml |
| Dilution range | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Note | For research use only |
| Application notes | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Expiration Date | 12 months from date of receipt. |
Example of nonspecific mouse IgG2b (MPC-11) biotin signal on human peripheral blood; surface staining, 16 µg/ml.
- Item 1 of 1
Llama IgG2 isotype control Biotin Antibody [orb343843]
DOT, ELISA, FC, WB
Biotin
Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to unconjugated llama IgG1 or IgG3 are observed. Biotin Conjugated Llama IgG2 was proven by Dot Blot.
Llama
100 μg 
Mouse IgG2b isotype control Biotin Antibody [orb343790]
FC, FLISA, IF
Biotin
Mouse IgG2b isotype control has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2b was proven by Dot Blot.
Mouse
100 μg
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