IGFBP6 Antibody

• Catalog Number: orb1247369
• Category: Antibodies
• Description: IGFBP6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RPQDVNRRDQQRNP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGFBP6
• UniProt ID: P24592
• NCBI: NP_002169.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IGFBP6 , insulin-like growth factor binding protei
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247369 (0.5 ug/ml) staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGFBP6 and probed with orb1247369 (mock transfection in first lane).

orb1247369 (5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.