IGF1R Rabbit Polyclonal Antibody

• Catalog Number: orb157658
• Category: Antibodies
• Description: IGF1R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Porcine, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF1R/CD221 beta chain (821-920/1367aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 69/150 kDa
• Target: IGF1R
• UniProt ID: P08069
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Insulin-like growth factor 1 receptor; CD221; CD22
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Mouse Spleen (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-IGF1R antibody (orb157658, Green), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: rabbit Anti-IGF1R orb157658, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-IGF1R (orb157658) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 69/150 kD, Observed band size: 69 kD.