IGF1R Rabbit Polyclonal Antibody

• Catalog Number: orb10881
• Category: Antibodies
• Description: IGF1R Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat, Sheep
• Reactivity: Canine, Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF-1R alpha chain (251-350/1367aa)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78/150 kDa
• Target: IGF1R
• UniProt ID: P08069
• RRID: AB_10750030
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Insulin-like growth factor 1 receptor; CD221; CD22
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hu, Wei et al. MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene Growth Factors, 32, 27-33 (2014)

PubMed: 24294913

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-IGF1R antibody (orb10881), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, catalog number: orb10881, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.