IGF I Rabbit Polyclonal Antibody

• Catalog Number: orb101730
• Category: Antibodies
• Description: IGF I Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF I (108-195/195aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 7.7/21 kDa
• Target: IGF1
• UniProt ID: P05019
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IBP1; IGF 1; IGF I; IGF-1; IGF IA; IGF IB; IGF1; I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

R Hernández, GG Castro, FM Rodríguez Insulin-like growth factors in cat placenta Analecta Veterinaria, (2025)

PubMed:

Sant'Ana, Fjf et al. Immunohistochemical expression of growth factors in the follicular wall of normal and cystic ovaries of sows Reprod Domest Anim, 50, 327-332 (2015)

PubMed: 25676567

Xu, Congyun et al. Effects of metformin on prostatic tissue of rats with metabolic syndrome and benign prostatic hyperplasia Int Urol Nephrol, 50, 611-617 (2018)

PubMed: 29460133

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IGF I) Polyclonal Antibody, Unconjugated (orb101730) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (human) Lysate at 40 ug, Primary: Anti-IGF1 (orb101730) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 7.7/21 kD, Observed band size: 25 kD.

Sample: Human IGF1 (Full Length) Overexpression Cell Lysate at 8 ug, Control (-) Cell Lysate at 8 ug, Primary: Anti-IGF 1 (orb101730) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD.

Tissue/cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-IGF I Polyclonal Antibody, Unconjugated (orb101730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.