IGF-1 Antibody

SKU: orb750208

Description

This antibody is specific to Insulin-like Growth Factor (IGF1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml (1)
• Reactivity: Human
• Application Notes:
  The optimal dilution of the IGF-1 antibody for each application should be determined by the researcher. 1. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SPM406
• Immunogen: Purified human IGF1 protein was used as the immunogen for this IGF-1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Quick Database Links

UniProt

P05019