IGF-1 Antibody / Insulin like Growth Factor 1

• Catalog Number: orb749359
• Category: Antibodies
• Description: This antibody is specific to IGF1 (Insulin like Growth Factor 1) and shows minimal cross-reaction with IGF11, Proinsulin, MSF, and Insulin. IGF1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF1B while isoform 2 is known as IGF1A. IGF1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of the IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M23
• Tested applications: FACS, IF, NeA
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Purified human IGF1 protein was used as the immunogen for this antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,In-vitro neutralization of biological activity of IGF-1 (order BSA/sodium azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This IGF1 antibody is available for research use only.
• Entrez: 3479
• Storage: Store the IGF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE Analysis of Purified, BSA-Free IGF1 Antibody (clone M23). Confirmation of Integrity and Purity of the Antibody.