IgA antibody

• Catalog Number: orb2276365
• Category: Antibodies
• Description: IgA antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-700R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P01877, P01876
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2
• Note: For research use only
• Application notes: Positive Control: Tonsil: A strong staining should be seen in a fraction of plasma cells. Negative Control: Tonsil: Lymphocytes should not stain for IgA. Cellular Localization: Cytoplasm, cell surface and secreted. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

IgA positive plasma cells are abundant in the duodenum mucosa.

IgA positive plasma cells are numerous in the tonsil.

Squamous cell carcinoma containing many IgA positive plasma cells in its stroma.