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Catalog Number | orb1272650 |
---|---|
Category | Antibodies |
Description | Ifng Antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Rat |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IFN-γ. Rat IFN-γ specific antibody was purified by affinity chromatography employing immobilized Rat IFN-γ matrix. |
Concentration | batch dependent |
Dilution range | To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | Ifng |
UniProt ID | P01581 |
NCBI | P01581 |
Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | IFNG2Interferon gammaIFN-gamma Read more... |
Note | For research use only |
Application notes | To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Rat IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IFN-gamma (orb1272661) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IFN-gamma.
ELISA, FC, ICC, IHC-P, IP, WB | |
Human, Primate | |
Monoclonal | |
Biotin |
ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Monkey, Primate | |
Mouse | |
Monoclonal | |
Biotin |
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