IFNAR1 Antibody

• Catalog Number: orb1273523
• Category: Antibodies
• Description: IFNAR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Immunogen: IFNAR1 (Ser535/539) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the human Phospho-Ser535, 539 Type I Interferon-a Receptor Subunit 1 (IFNAR1).
• Concentration: batch dependent
• Dilution range: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110-130
• Target: IFNAR1
• UniProt ID: P17181
• NCBI: P17181
• Storage: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
• Note: For research use only
• Application notes: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Expiration Date: 12 months from date of receipt.

Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).