IFN gamma antibody

• Catalog Number: orb345094
• Category: Antibodies
• Description: IFN gamma antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 145 amino acids of mature chicken IFN gamma.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, WB: 1:500-1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for chicken IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from chicken based on 100% homology; ring-necked pheasant, turkey, quail, and junglefowl based on 91-97% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: D3GGX1
• NCBI: NP_990480.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interferon gamma, IFNG, rabbit anti-IF
• Note: For research use only
• Application notes: This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.7 kDa in size corresponding to chicken IFN gamma by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-chicken IFN gamma antibody shows detection of recombinant chicken IFN gamma at 16.7 kDa, raised in yeast. Primary antibody was diluted to 1 µg/mL. 3% BSA from Biorbyt's Bovine Serum Albumin Solution was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye 800) was used at 1:20000.