IFN beta antibody

• Catalog Number: orb345259
• Category: Antibodies
• Description: IFN beta antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 164 amino acids of the mature swine IFN beta protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, WB: 1:500-1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for swine IFN beta protein. A BLAST analysis was used to suggest cross-reactivity with IFN beta from Sus scrofa (pig), Potamochoerus larvatus koiropotamus, Potamochoerus larvatus (Bushpig), and Phacochoerus africanus (warthog) sources based on 100% homology with the immunizing sequence. Cross-reactivity with IFN beta from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q29412
• NCBI: NP_001003923.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Interferon Beta Antibody, rabbit anti-
• Note: For research use only
• Application notes: This protein A purified IFN-beta antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.5 kDa in size corresponding to swine IFN beta by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's protein-A purified anti-swine IFN beta antibody shows detection of recombinant Swine IFN beta at about 19.5 kDa raised in yeast. Membrane was blocked with 3% BSA (diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800® Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.