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Catalog Number | orb1229843 |
---|---|
Category | Proteins |
Description | IFN alpha-2a Recombinant Protein |
Tag | Tag Free |
Dilution range | This recombinant protein can be used for biological assays. For research use only. |
Form/Appearance | Lyophilized |
Purity | Greater than 95% as determined by reducing SDS-PAGE. Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
MW | 19.24 kD |
Target | IFNA2 |
UniProt ID | P01563 |
Protein Sequence | Cys24-Glu188 |
Source | E. coli |
Biological Activity | Specific Activity is greater than 1.0 x 108 IU/ mg as determined in a viral resistance assay using VSV-WISH cells. |
NCBI | P01563 |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
Alternative names | Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha- Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for biological assays. For research use only. |
Expiration Date | 6 months from date of receipt. |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by both(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
19.24 KDa | |
E. Coli |
CLIA, ELISA, LF, WB | |
> 90% pure by SDS-PAGE. Ligand affinity purified. | |
This recombinant protein contains full length of Human IFN-alpha 2A with a MW of 20.7 kDa. It is recognized by an anti-human IFN-alpha 2A mAb from R&D systems (Cat#93452) in ELISA tests. | |
E. coli |
> 95% by SDS-PAGE. | |
KMP1620, Recombinant Human IFNα2a/IFN-alpha 2a Protein is produced by E. coli expression system. The target protein is expressed with sequence (Cys24-Glu188) of human IFNα2a/IFN-alpha 2a (Accession #P01563) fused with an initial Met at the N-terminus. |
> 90% as determined by SDS-PAGE | |
21.6 kDa |
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