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Catalog Number | orb345212 |
---|---|
Category | Antibodies |
Description | IDO2 antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1HC |
Tested applications | ELISA, IF, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:5000 - 1:50000, IF: 1:50-1:100, WB: 1:500-1:1500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-IDO2 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. IDO2 antibody is specific for mouse IDO2 protein. Cross-reactivity with IDO2 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q8R0V5 |
NCBI | NP_666061.3 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse anti-IDO2 Antibody, Indoleamine 2,3-dioxygen Read more... |
Note | For research use only |
Application notes | Anti-IDO2 antibody has been tested in western blot and is suitable for use in ELISA and IF. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.2 kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms.
ELISA, FC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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