ICOSLG antibody

• Catalog Number: orb412540
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ICOSLG
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human CD275
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ICOSLG
• Entrez: 50723, 23308
• UniProt ID: O75144, Q9JHJ8
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-B7H2 antibody, anti-B7RP1 antibody, anti-ICOS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD275 expression in HL60 (A), mouse brain (B), rat spleen (C) whole cell lysates. (Predicted band size: 20; 33; 34 kD; Observed band size: 60 kD)

Immunohistochemical analysis of CD275 staining in human skin cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.