Icosl Antibody

• Catalog Number: orb1671835
• Category: Antibodies
• Description: Icosl Antibody
• Clonality: Recombinant
• Clone Number: HK5.3
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Icosl
• UniProt ID: Q9JHJ8
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7RP-1, B7-related protein 1, ICOS ligand, CD275,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-ICOSL (inducible T-cell costimulator ligand) antibody HK5.3. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of HK5.3 (orb1671835) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing weak membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671835- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Binding curve of anti-ICOSL antibody HK5.3 (orb1671835) to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.1 ng/ml was performed using orb1671835. For detection- a 1:4000 dilution of HRP-loat anti-ribody was used.