ICAM1 Rabbit Polyclonal Antibody

• Catalog Number: orb10334
• Category: Antibodies
• Description: ICAM1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD54 (201-300/537aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58/100 kDa
• Target: ICAM1
• UniProt ID: P05362
• RRID: AB_10751662
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BB2; CD54 antigen; ICAM 1; ICAM-1; Cell surface gl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): A431 cells (blue). Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4℃. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: HUVEC cells (blue). Primary Antibody: Rabbit Anti-CD54 antibody (orb10334), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10334, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Blank control: mouse thymouses (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA (green).

Sample: Hela (Human) Cell Lysate at 30 ug, Hela KO ICAM1 (Human) Cell Lysate at 30 ug, Primary: Anti-ICAM1/CD54 (orb10334) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.