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Catalog Number | orb180715 |
---|---|
Category | Antibodies |
Description | ICAM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, IHC-Fr, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 57825 MW |
UniProt ID | P05362 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Intercellular adhesion molecule 1;ICAM-1;Major gro Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-ICAM1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ICAM1 using anti-ICAM1 antibody.Lane 1:HEPG2 Cell;2:K562 Cell;3:A549 Cell;4:HT1080 Cell.
IF analysis of ICAM1 using anti-ICAM1 antibody.ICAM1 was detected in paraffin-embedded section of human tonsil tissues.
IF analysis of ICAM1 using anti-ICAM1 antibody.ICAM1 was detected in paraffin-embedded section of human Lung cancer tissues.
IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues.
FC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Bovine, Porcine | |
Goat, Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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