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Item 1 of 6
Item 1 of 6
ICAM-1 / CD54 (Extracellular)
Catalog Number: orb1294365
| Catalog Number | orb1294365 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ICAM-1 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human ICAM-1 / CD54 |
| Dilution range | Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
| Conjugation | Unconjugated |
| Target | ICAM-1 / CD54 (Extracellular) |
| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ICAM-1 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 Anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 Anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ICAM-1/CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
