I kappa B-alpha (phospho-Tyr42) antibody

• Catalog Number: orb769255
• Category: Antibodies
• Description: Rabbit polyclonal antibody to I kappa B-alpha (phospho-Tyr42)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr42. AA range:9-58
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 35
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-NFKBIA antibody, anti-IKBA antibody, anti-MAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IkappaB-alpha (Phospho-Tyr42) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-alpha (Phospho-Tyr42) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night.