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Catalog Number | orb345458 |
---|---|
Category | Antibodies |
Description | Hus1B antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human Hus1B protein. |
Concentration | 1.75 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IP: 1:100, WB: 1:500 - 1:5,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity-purified antibody is directed against human Hus1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human. Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | Q8NHY5 |
NCBI | 31077215 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-HUS1B antibody, Checkpoint protein HUS Read more... |
Note | For research use only |
Application notes | Anti-HUS1B antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to Hus1B protein by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's affinity purified anti-Hus1B antibody shows detection of a 36 kDa band [arrow] corresponding to Hus1B in a HeLa cell lysate (p/n orb348668). The staining pattern in 293 cells (p/n orb348669) is less clear, showing a predominant band at 39 kDa.
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