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Item 1 of 6
Item 1 of 6
HuR / ELAVL1 Antibody, KO Validated
Catalog Number: orb1274439
| Catalog Number | orb1274439 |
|---|---|
| Category | Antibodies |
| Description | HuR / ELAVL1 Antibody, KO Validated |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR / ELAVL1 (NP_001410.2). |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 36kDa |
| Target | ELAVL1 |
| UniProt ID | Q15717 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like p Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (orb1274439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Western blot analysis of extracts from normal (control) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (orb1274439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HuR/ELAVL1 antibody (orb1274439) at dilution of 1:100. Blue: DAPI for nuclear staining.
- Item 1 of 8
HuR / ELAVL1 Antibody, KO Validated [orb1274490]
IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl
