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Item 1 of 3
| Catalog Number | orb257924 |
|---|---|
| Category | Proteins |
| Description | Human VEGF165, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
| Reactivity | Human |
| Tag | N-10×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 21.1 kDa |
| Target | VEGF165 |
| UniProt ID | P15692 |
| Source | Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Ala 27 - Arg 191 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_001165097.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Kerros, Celine et al. Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells J Biol Chem, 292, 10295-10305 (2017)
SDS-PAGE analysis of Human VEGF protein
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. orb257924) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
- Item 1 of 4
Human VEGF165 Protein [orb257923]
Unconjugated
95%
19.2 kDa
Human VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
1 mg, 50 μg, 20 μg - Item 1 of 1
Human VEGF165 Protein, hFc Tag [orb2321384]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg - Item 1 of 1
Recombinant human VEGF-165 protein (Active, HEK293) [orb1817182]
> 95% as determined by SDS-PAGE
39-45 kDa
50 μg, 10 μg - Item 1 of 1
Recombinant human VEGF165 protein, C-His [orb1516905]
> 95% as determined by SDS-PAGE
20 kDa
500 μg, 100 μg 
Human VEGF165 Protein [orb1289549]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μg
