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Human VEGF165 Protein

Catalog Number: orb257923

DispatchUsually dispatched within 5-10 working days
$ 490.00
Catalog Numberorb257923
CategoryProteins
DescriptionHuman VEGF165, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
ReactivityHuman
TagNative
Form/AppearancePowder
Purity95%
ConjugationUnconjugated
MW19.2 kDa
TargetVEGF165
UniProt IDP15692
SourceHuman VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
Expression SystemHEK293
Biological OriginHuman
Biological ActivityAvastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Expression RegionAla 27 - Arg 191
Endotoxins0.01 EU per μg
NCBINP_001165097.1
Storage-20°C to -70°C for 24 months in lyophilized state; -70°C for 24 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesRP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
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NoteFor research use only
Application notesThis protein carries no "tag". The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).
Expiration Date6 months from date of receipt.
Human VEGF165 Protein

SDS-PAGE analysis of Human VEGF protein

Human VEGF165 Protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Human VEGF165 Protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Human VEGF165 Protein

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165, premium grade (Cat. No. orb257923) with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Human VEGF165 Protein [orb257924]

    Unconjugated

    90%

    21.1 kDa

    Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).

    1 mg, 50 μg
  • Human VEGF165 Protein, hFc Tag [orb2321384]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg
  • Recombinant human VEGF-165 protein (Active, HEK293) [orb1817182]

    > 95% as determined by SDS-PAGE

    39-45 kDa

    50 μg, 10 μg
  • Recombinant human VEGF165 protein, C-His [orb1516905]

    > 95% as determined by SDS-PAGE

    20 kDa

    500 μg, 100 μg
  • Human VEGF165 Protein [orb1289549]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    10 μg, 50 μg, 500 μg