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Catalog Number | orb1289549 |
---|---|
Category | Proteins |
Description | Recombinant Human VEGF165 Protein |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | VEGFA |
Entrez | 7422 |
UniProt ID | P15692 |
Protein Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
Source | Human cells |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | VEGF; Vascular endothelial growth factor A; VEGF-A Read more... |
Note | For research use only |
Application notes | Please minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
19.2 kDa | |
Human VEGF165, premium grade (orb257923) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
Unconjugated | |
90% | |
21.1 kDa | |
Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGF165-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE | |
39-45 kDa |
> 95% as determined by SDS-PAGE | |
20 kDa |