Human VEGF165 Protein, His Tag

• Catalog Number: orb257924
• Category: Proteins
• Description: Human VEGF165, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 21.1 kDa
• Target: VEGF165
• UniProt ID: P15692
• Source: Human VEGF165, His Tag (orb257924) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ala 27 - Arg 191
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001165097.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Kerros, Celine et al. Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells J Biol Chem, 292, 10295-10305 (2017)

PubMed: 28468826

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. orb257924) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).