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Item 1 of 3
Item 1 of 3
| Catalog Number | orb257918 |
|---|---|
| Category | Proteins |
| Description | Human VEGF121 Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9). |
| Reactivity | Human |
| Tag | Native |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 14.1 kDa |
| Target | VEGF121 |
| UniProt ID | P15692 |
| Source | Human VEGF121 Protein, premium grade (orb257918) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human VEGF121 Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested). |
| Expression Region | Ala 27 - Arg 147 |
| Endotoxins | 0.1 EU per μg |
| NCBI | NP_001165099.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF Read more... |
| Note | For research use only |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 15-16 kDa and 17-20 kDa under reducing (R) condition, and 30-40 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Stafford, Ryan L. et al. In vitro Fab display: a cell-free system for IgG discovery Protein Eng Des Sel, 27, 97-109 (2014)
SDS-PAGE analysis of Human VEGF protein
Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations
Immobilized Human VEGF121 Protein, premium grade (Cat. No. orb257918) at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested).
- Item 1 of 3
Human VEGF121 Protein [orb334936]
Unconjugated
95%
14.9 kDa
Human VEGF121, His Tag (orb334936) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
50 μg, 1 mg - Item 1 of 1
Human VEGF121 Protein, hFc Tag [orb2321385]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg 
VEGF121 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960084]
98.00%
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgVEGF121 Protein, Human, Recombinant (His & Avi) [orb1960085]
98.00%
17 kDa (predicted). Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
