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Catalog Number | orb1496221 |
---|---|
Category | Proteins |
Description | Human VEGF110 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 136 (Accession # NP_001165097.1). |
Reactivity | Human |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 14.6 kDa |
Target | VEGF110 |
Source | Human VEGF110 Protein, His Tag (orb1496221) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 136 (Accession # NP_001165097.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human VEGFR1/R2 captured on Protein A Chip can bind Human VEGF110 Protein, His Tag with an affinity constant of 13.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Ala 27 - Arg 136 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001165097.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.6 kDa. The protein migrates as 15 kDa and 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human VEGFR1/R2 captured on Protein A Chip can bind Human VEGF110 Protein, His Tag (Cat. No. orb1496221) with an affinity constant of 13.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).