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Catalog Number | orb18510 |
---|---|
Category | Proteins |
Description | Human VEGF R3, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 111.2 kDa |
Target | VEGF R3 |
UniProt ID | P35916 |
Source | Human VEGF R3, Fc Tag (orb18510) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Tyr 25 - Ile 776 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_002011.2 |
RRID | AB_10746635 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FL Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human VEGF R3 protein
Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).