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Human VEGF-D Protein
Catalog Number: orb257928
| Catalog Number | orb257928 |
|---|---|
| Category | Proteins |
| Description | Human VEGF-D, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948). |
| Reactivity | Human |
| Tag | C-9×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 13.4 kDa |
| Target | VEGF-D |
| UniProt ID | O43915 |
| Source | Human VEGF-D, His Tag (orb257928) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Phe 93 - Ser 201 |
| Endotoxins | 1.0 EU per μg |
| NCBI | AAH27948 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | FIGF,VEGFD Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human VEGF-D protein
Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Human VEGFD Protein, hFc Tag [orb2321358]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.3 kDa after removal of the signal peptide. The apparent molecular mass of VEGFD-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg 
Human VEGF-D protein [orb245335]
Greater than 90% as determined by SDS-PAGE.
40.1 kDa
E.coli
20 μg, 100 μg, 1 mgHuman FIGF protein [orb391743]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgRecombinant human VEGFD Protein,C-His (HEK293) [orb2328527]
> 85% as determined by SDS-PAGE
20 μg, 50 μg, 100 μg
