Human VD3(Vitamin D3) ELISA Kit

• Catalog Number: orb1736531
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 6.25-400 ng/mL
• Sensitivity: 2.14 ng/mL
• Alternative names: Cholecalciferol; Toxiferol; Activated 7-Dehydrocho
• Note: For research use only
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.