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| Catalog Number | orb1736531 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 6.25-400 ng/mL |
| Concentration | 400 ng/mL |
| Sensitivity | 2.07 ng/mL |
| Alternative names | Cholecalciferol; Toxiferol; Activated 7-Dehydrocho Read more... |
| Note | For research use only |
| Application notes | standard: 400 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
