Human V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) ELISA Kit

• Catalog Number: orb778241
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog(KRAS).
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Assay Time: 3.5h
• Concentration: 20 ng/mL
• Sensitivity: 0.127 ng/mL
• Alternative names: C-K-RAS ELISA Kit, K-RAS2A ELISA Kit, K-RAS2B ELIS
• Note: For research use only
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRAS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRAS in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.