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Catalog Number | orb392666 |
---|---|
Category | Proteins |
Description | Recombinant human UBE2M protein is produced by our E. coli expression system and the target gene encoding Met1-Lys183 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 2mM DTT, 150mM NaCl, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | UBE2M |
Entrez | 9040 |
UniProt ID | P61081 |
Protein Sequence | MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 50mM HEPES, 2mM DTT, 150mM NaCl, 10% Glycerol, pH 7.5. |
Alternative names | UBC12; NEDD8-conjugating enzyme Ubc12; NEDD8 carri Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
22.8 kDa | |
E.Coli |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses. | |
20.9 kDa | |
E. coli. |
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