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Catalog Number | orb1476709 |
---|---|
Category | Proteins |
Description | Recombinant Human Trophoblast glycoprotein(TPBG),partial (Active) |
Tag | N-terminal 10xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 38.9 kDa |
UniProt ID | Q13641 |
Protein Sequence | SSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS |
Protein Length | Partial |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.230-1.519 ng/mL. |
Expression Region | 32-355aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Alternative names | (5T4 oncofetal antigen)(5T4 oncofetal trophoblast Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
> 95% by SDS-PAGE. | |
KMP1772, Recombinant Human TPBG Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ser32-Ser355) of human TPBG (Accession #Q13641) fused with a 6×His Tag at the C-terminus. |
Unconjugated | |
90% | |
62.2 kDa | |
Mouse TPBG Protein, Fc Tag (orb1733266) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 361 (Accession # Q9Z0L0-1). |
Unconjugated | |
90% | |
37.7 kDa | |
Rat TPBG Protein, His Tag (orb1733279) is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 361 (Accession # Q5PQV5). |
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