Human TNFRSF5(Tumor Necrosis Factor Receptor Superfamily, Member 5) ELISA Kit

• Catalog Number: orb1950103
• Category: Assays and Kits
• Description: Human TNFRSF5(Tumor Necrosis Factor Receptor Superfamily, Member 5) ELISA Kit
• Reactivity: Human
• Range: 15.63-1000 pg/mL
• UniProt ID: P25942
• Sensitivity: 4.12 pg/mL
• Alternative names: CD40; CDW40; Bp50; P50; B-cell surface antigen CD4
• Note: For research use only
• Application notes: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFRSF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFRSF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFRSF5 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.