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Catalog Number | orb1743387 |
---|---|
Category | Proteins |
Description | Recombinant Human TNFRSF1B Protein with C-terminal human Fc tag |
Reactivity | Human |
Tag | C-Human Fc Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1B-hFc is approximately 55-70kDa due to glycosylation. |
Target | TNFRSF1B |
UniProt ID | P20333 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | CD120b;p75;p75TNFR;TBPII;TNF-R-II;TNF-R75;TNFBR;TN Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human B7-2 Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CTLA4, mFc-His tagged protein orb689378 in a linear range of 0.048-2.694 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein (orb689458) can bind Human CD28, mFc-His tagged protein orb689375 in a linear range of 62.5-4000 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-2, hFc tagged protein (orb689458) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.