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Catalog Number | orb359192 |
---|---|
Category | Proteins |
Description | Recombinant human TNFA active protein |
Tag | N-terminal 6xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | > 97% as determined by SDS-PAGE and HPLC. |
MW | 18.3 kDa |
UniProt ID | P01375 |
Protein Sequence | MHHHHHH+VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL |
Protein Length | Partial |
Source | E.Coli |
Biological Origin | Homo sapiens (Human) |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. |
Expression Region | 77-233aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered PBS, pH 7.0 |
Alternative names | Cachectin, Tumor necrosis factor ligand superfamil Read more... |
Note | For research use only |
Application notes | This is His protein |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
17.5 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
18.5 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
21.8 kDa | |
E.coli |
> 98% as determined by SDS-PAGE and HPLC. | |
16.9 kDa | |
E.Coli |
> 98% as determined by SDS-PAGE and HPLC. | |
17.5 kDa | |
E.Coli |