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Human TNF-beta / Lymphotoxin-alpha Protein, His Tag (MALS verified)
Catalog Number: orb1733281
| Catalog Number | orb1733281 |
|---|---|
| Category | Proteins |
| Description | Human TNF-beta Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374). |
| Reactivity | Human |
| Tag | C-10×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 20.5 kDa |
| Target | TNF-beta |
| UniProt ID | P01374 |
| Source | Human TNF-beta Protein, His Tag (orb1733281) is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Immobilized Human TNFR1, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag with a linear range of 1-20 ng/mL (QC tested). |
| Expression Region | Leu 35 - Leu 205 |
| Endotoxins | 1.0 EU per μg |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Immobilized Human TNFR1, Fc Tag (Cat. No. orb257878) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. orb1733281) with a linear range of 1-20 ng/mL (QC tested).
