Human TGF-Beta 1 / TGFB1 Protein

• Catalog Number: orb257996
• Category: Proteins
• Description: Human TGFB1, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 12.8 kDa (monomer)
• Target: TGF-beta 1
• UniProt ID: P01137
• Source: Human TGFB1, premium grade (orb257996) is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human TGFB1, premium grade at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag with a linear range of 0.2-2.5 ng/mL (QC tested).
• Expression Region: Ala 279 - Ser 390
• Endotoxins: 0.1 EU per μg
• NCBI: NP_000651.3
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 0.085% TFA in 30% ACN
• Alternative names: CEDLAP,TGF-beta 1,TGFB1,DPD1,TGF-beta-1,TGFB
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 12.8 kDa (monomer). the protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Cirka, Heather et al. Active Traction Force Response to Long-Term Cyclic Stretch Is Dependent on Cell Pre-stress Biophys J, 110, 1845-1857 (2016)

PubMed: 27119644

SDS-PAGE analysis of Human TGF-beta 1 protein

Immobilized Human TGFB1, premium grade (Cat. No. orb257996) at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag (Cat. No. orb257866) with a linear range of 0.2-2.5 ng/mL (QC tested).