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Catalog Number | orb1088171 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Tocopherol(TCPa) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Tocopherol(TCPa). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Tocopherol(TCPa) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 1.57-100 μg/mL |
Sensitivity | 0.46 μg/mL |
Protein Length | 2h |
Alternative names | VE, Vitamin E, α-TCP, α-Tocopherol Read more... |
Note | For research use only |
Sample Types | Serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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