Human Sonic Hedgehog / Shh Protein

• Catalog Number: orb257838
• Category: Proteins
• Description: Human Sonic Hedgehog, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 20.4 kDa
• Target: Sonic Hedgehog
• UniProt ID: Q15465
• Source: Human Sonic Hedgehog, His Tag, premium grade (orb257838) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Cys 24 - Gly 197
• Endotoxins: 0.2 EU per μg
• NCBI: NP_000184
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Sonic Hedgehog protein