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Human Sonic Hedgehog Protein, His Tag
Catalog Number: orb257838
| Catalog Number | orb257838 |
|---|---|
| Category | Proteins |
| Description | Human Sonic Hedgehog, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184). |
| Reactivity | Human |
| Tag | C-6×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 20.4 kDa |
| Target | Sonic Hedgehog, Shh |
| UniProt ID | Q15465 |
| Source | Human Sonic Hedgehog, His Tag, premium grade (orb257838) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Cys 24 - Gly 197 |
| Endotoxins | 0.2 EU per μg |
| NCBI | NP_000184 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Sonic Hedgehog protein
Human SHH Protein, His Tag [orb2701586]
> 97%, determined by SDS-PAGE
This protein contains the amino acid sequence (Cys 198-Ser 462) of human SHH (Q15465), that is Sonic hedgehog protein C-product, was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells.
20 μg, 5 μgHuman SHH Protein, His Tag [orb2701587]
> 95%, determined by SDS-PAGE
This protein contains the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465), that is Sonic hedgehog protein N-product, was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
20 μg, 5 μgHuman FGF-4 Protein, His Tag [orb1733261]
Unconjugated
90%
19.8 kDa
Human FGF-4 Protein, His Tag (orb1733261) is expressed from E. coli cells. It contains AA Ser 54 - Leu 206 (Accession # P08620-1).
1 mg, 100 μg
