Human Soluble Cluster of Differentiation 40 Ligand (sCD40L) ELISA Kit

• Catalog Number: orb866982
• Category: Assays and Kits
• Description: Sandwich Human sCD40L ELISA Kit
• Tested applications: ELISA
• Reactivity: Human
• Range: 15.63-1000 pg/mL
• Assay Time: 3.5h
• Concentration: 1000 pg/mL
• Sensitivity: 6.6 pg/mL
• Alternative names: CD154 ELISA Kit, TRAP ELISA Kit, HIGM1 ELISA Kit,
• Note: For research use only
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sCD40L in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.