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Catalog Number | orb753163 |
---|---|
Category | Tools |
Description | SIGLEC10 Human produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 678 amino acids (17-455 a.a.) and having a molecular mass of 75.6kDa. SIGLEC10 is expressed with a 239 hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 85.0% as determined by SDS-PAGE. |
Protein Sequence | MDGRFWIRVQ ESVMVPEGLC ISVPCSFSYP RQDWTGSTPA YGYWFKAVTE TTKGAPVATNHQSREVEMST RGRFQLTGDP AKGNCSLVIR DAQMQDESQY FFRVERGSYV RYNFMNDGFFLKVTALTQKP DVYIPETLEP GQPVTVICVF NWAFEECPPP SFSWTGAALS SQGTKPTTSHFSVLSFTPRP QDHNTDLTCH VDFSRKGVSA QRTVRLRVAY APRDLVISIS RDNTPALEPQPQGNVPYLEA QKGQFLRLLC AADSQPPATL SWVLQNRVLS SSHPWGPRPL GLELPGVKAGDSGRYTCRAE NRLGSQQRAL DLSVQYPPEN LRVMVSQANR TVLENLGNGT SLPVLEGQSLCLVCVTHSSP PARLSWTQRG QVLSPSQPSD PGVLELPRVQ VEHEGEFTCH ARHPLGSQHVSLSLSVHYKK GLISTAFSNL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISRTPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLNGKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPSDIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH |
Source | Sf9, Baculovirus cells |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | The SIGLEC10 solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
Alternative names | SIGLEC10, PRO940, SLG2, SIGLEC-10, Sialic acid-bin Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 12 months from date of receipt. |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.0 kDa after removal of the signal peptide.The apparent molecular mass of SIGLEC10-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 95% by SDS-PAGE. | |
KMP1825, Recombinant Human Siglec10 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (SIGLEC10(Met17-Thr546)) of human Siglec10 (Accession #) fused with a His Tag at the C-terminus. |
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