Human Siglec-10 Protein (L118A, G120A, E201A) Tag

• Catalog Number: orb1291809
• Category: Proteins
• Description: Human Siglec-10, Fc (L118A, G120A, E201A) Tag is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
• Reactivity: Human
• Tag: C-Fc(A3)
• Form/Appearance: Powder
• Purity: 85%
• Conjugation: Unconjugated
• MW: 84.6 kDa
• Target: Siglec-10
• UniProt ID: Q96LC7
• Protein Sequence: NP_149121.2
• Source: Human Siglec-10, Fc (L118A, G120A, E201A) Tag (orb1291809) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Met 17 - Thr 546
• Endotoxins: 1.0 EU per μg
• NCBI: NP_149121.2
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
• Expiration Date: 6 months from date of receipt.