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Catalog Number | orb707050 |
---|---|
Category | Proteins |
Description | Recombinant Human SHH Protein is produced by E.coli expression system and the target gene encoding Cys24-Gly197 (Cys24Ile-Ile) is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SHH |
Entrez | 6469 |
UniProt ID | Q15465 |
Protein Sequence | MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. |
Alternative names | Sonic hedgehog protein protein, SHH protein, HHG-1 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
20.4 kDa | |
Human Sonic Hedgehog, His Tag, premium grade (orb257838) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184). |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
> 98% as determined by SDS-PAGE and HPLC. | |
19.8 kDa | |
E.Coli |
Greater than 95% as determined by reducing SDS-PAGE. | |
20.4 KDa | |
Mammalian |