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Human SHH protein (Active)

Catalog Number: orb359257

DispatchUsually dispatched within 1-2 weeks
$ 210.00
Catalog Numberorb359257
CategoryProteins
DescriptionRecombinant human SHH active protein
TagTag-Free
Form/AppearanceLyophilized powder
Purity> 98% as determined by SDS-PAGE and HPLC.
MW19.8 kDa
UniProt IDQ15465
Protein SequenceIVIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Protein LengthPartial
SourceE.Coli
Biological OriginHomo sapiens (Human)
Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 × 103 IU/mg.
Expression Region25-197aa
EndotoxinsLess than 1.0 EU/µg as determined by LAL method.
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Buffer/PreservativesLyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Alternative namesSHH,
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NoteFor research use only
Application notesWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Expiration Date6 months from date of receipt.
Human SHH protein (Active)

SDS-PAGE analysis of Human SHH protein (Active)

  • Recombinant Human Sonic hedgehog protein(SHH) (Active) [orb1650543]

    100 μg, 5 μg, 25 μg, 1 mg, 250 μg, 500 μg
  • Recombinant Human Sonic HedgeHog (Shh) [orb1494940]

    > 98% by SDS-PAGE and HPLC analyses.

    Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

    Escherichia coli

    1 mg, 25 μg, 5 μg
  • RecombinantIHH,Human [orb1494835]

    > 95% as analyzed by SDS-PAGE.

    20 kDa, observed by reducing SDS-PAGE.

    Escherichia coli.

    50 μg, 10 μg, 1 mg
  • RecombinantShh,Mouse(CHO-expressed) [orb1494667]

    > 95% as analyzed by SDS-PAGE and HPLC.

    20 kDa, observed by non-reducing SDS-PAGE.

    CHO

    50 μg, 10 μg, 1 mg